COX4 (8D8) Monoclonal Antibody
- Size: 100ul
- Application: WB, IHC-P, IF(IHC-P)
- Reactivity: Human, Rat
- Predicted Reactivity:
-
Datasheet
Tech Support
IMAGES
Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX4 Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with COX4 Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-CY3) for 90 minutes, and DAPI for nuclei staining.
Jurkat cell lysates probed with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37°C.
HepG2 cell lysates probed with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:300 overnight at 4°C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37°C.
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
COX4 (8D8) Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Mouse |
| SOURCE |
Recombinant human COX4 protein |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein G. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
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| SUBCELLULAR LOCATION |
|
| SYNONYMS |
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4I1 |
| BACKGROUND |
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. |
| APPLICATION DILUTION |
WB(1:300-1000), IHC-P(1:200-400), IF(IHC-P)(1:50-200) |
