ChAT Polyclonal Antibody
- Size: 100ul
- Application: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
- Reactivity: Human, Mouse, Rat, Dog
- Predicted Reactivity: Pig
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Datasheet
Tech Support
IMAGES
SHSY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20_, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ChAT Polyclonal Antibody(bs-0042R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
ChAT Polyclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
KLH conjugated synthetic peptide derived from human ChAT |
| IMMUNOGEN RANGE |
101-200/748 |
| CLONALITY |
Polyclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Cytoplasm, Nucleus |
| SYNONYMS |
CMS1A; CMS1A2; CHOACTASE; Choline O-acetyltransferase; ChAT; Choline acetylase |
| BACKGROUND |
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. |
| APPLICATION DILUTION |
ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200) |
