CDC123 Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM
- Reactivity: Human, Mouse
- Predicted Reactivity:
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Datasheet
Tech Support
IMAGES
Flow cytometric analysis of CDC123 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54706R, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
CDC123 Monoclonal Antibody |
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CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within C-terminal human CDC123. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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| GENE ID |
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| SUBCELLULAR LOCATION |
Cytoplasm |
| SYNONYMS |
C10orf7 antibody, CD123_HUMAN antibody, CDC123 antibody, Cell division cycle 123 antibody, Cell division cycle 123 homolog antibody, Cell division cycle protein 123 homolog antibody, D123 antibody, HT 1080 antibody, HT-1080 antibody, Protein D123 antibody, PZ32 antibody |
| BACKGROUND |
Required for S phase entry of the cell cycle.The eukaryotic cell division cycle consists of a number of gene-controlled sequences that involve cyclin dependent kinases (Cdks) and cell division control (Cdc) proteins. Cdc123 (Cell division cycle protein 123), also known as D123, is a 336 amino acid cytoplasmic protein that is involved in cell cycle control. Widely expressed with high expression in thymus, spleen, ovary, testis, small intestine and leukocytes, Cdc123 functions to destabilize Chfr (checkpoint with forkhead and ring finger domain) proteins which, when accumulated, block the G to S phase transition. Cdc123 prevents the Chfr proteins from collecting in the cell, thereby allowing the cell to enter the S phase. Due to its role in cell cycle control, Cdc123 is thought to be a basal marker for luminal breast cancers. |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100) |