BCL2L12 Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IHC-P, IF(ICC)
- Reactivity: Human
- Predicted Reactivity:
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Datasheet
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IMAGES
SW620 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (BCL2L12) Monoclonal Antibody, Unconjugated (bsm-54682R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (BCL2L12) Monoclonal Antibody, Unconjugated (bsm-54682R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (BCL2L12) Monoclonal Antibody, Unconjugated (bsm-54682R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BCL2L12 Monoclonal Antibody, Unconjugated (bsm-54682R) at 1:400 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BCL2L12 Monoclonal Antibody, Unconjugated (bsm-54682R) at 1:800 for 30 minutes at room temperature, DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
BCL2L12 Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within C-terminal human BCL2L12 |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
|
| SUBCELLULAR LOCATION |
Nucleus, Membrane |
| SYNONYMS |
B2L12_HUMAN antibody, BCL 2 like 12 (proline rich) antibody, BCL 2 like 12 antibody, BCL 2 like 12 isoform 1 antibody, Bcl 2 like 12 protein antibody, Bcl 2 related proline rich protein antibody, Bcl-2-like protein 12 antibody, Bcl-2-related proline-rich protein antibody, BCL2 like 12 (proline rich) antibody, BCL2 like 12 antibody, BCL2 like 12 isoform 1 antibody, Bcl2 like 12 protein antibody, Bcl2 related proline rich protein antibody, Bcl2-L-12 antibody, BCL2L12 antibody, BPR antibody, MGC120313 antibody, MGC120314 antibody, MGC120315 antibody |
| BACKGROUND |
Expressed mainly in breast, thymus, prostate, fetal liver, colon, placenta, pancreas, small intestine, spinal cord, kidney, and bone marrow and to a lesser extent in many other tissues. Isoform 2 is primarily expressed in skeletal muscle. |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200) |