Arg2 Monoclonal Antibody
- Size: 100ul
- Application: WB, FCM, IHC-P, IF(ICC)
- Reactivity: Human
- Predicted Reactivity:
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Datasheet
Tech Support
IMAGES
RWPE-1 cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Arg2) monoclonal Antibody, Unconjugated (bsm-54747R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Arg2 Monoclonal Antibody, Unconjugated (bsm-54747R) at 1:200 for 30 minutes at room temperature, DAB staining.
293 cells were fixed,permeabilized and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Arg2 Monoclonal Antibody(bsm-54747R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (red).
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Arg2 Monoclonal Antibody, Unconjugated (bsm-54747R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human prostate cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Arg2 Monoclonal Antibody, Unconjugated (bsm-54747R) at 1:50 for 30 minutes at room temperature, DAB staining.
SW620 cell;4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Arg2) monoclonal Antibody, Unconjugated (bsm-54747R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
Arg2 Monoclonal Antibody |
|
CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
Synthetic Peptide within C-terminal Human Arg2. |
| IMMUNOGEN RANGE |
|
| CLONALITY |
Monoclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
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| SUBCELLULAR LOCATION |
Mitochondrion |
| SYNONYMS |
ARG2 antibody, ARGI2_HUMAN antibody, Arginase II mitochondrial antibody, Arginase type II antibody, Arginase-2 antibody, arginase-2, mitochondrial antibody, Kidney arginase antibody, Kidney type arginase antibody, Kidney-type arginase antibody, L arginine amidinohydrolase antibody, L arginine ureahydrolase antibody, mitochondrial antibody, Non hepatic arginase antibody, Non-hepatic arginase antibody, Nonhepatic arginase antibody, Type II arginase antibody |
| BACKGROUND |
Arginase I (also designated liver-type arginase), which is expressed almost exclusively in the liver, catalyzes the conversion of arginine to ornithine and urea (1). The human arginase I gene, which maps to chromosome 6q23, encodes a 322 amino acid protein. Arginase I exists as a homotrimeric protein and contains a binuclear manganese cluster (2-4). Arginase II catalyzes the same reaction as arginase I, but differs in its tissue specificity and subcellular location (5,6). Specifically, arginase II localizes to the mitochondria (5,6). Arginase II is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase I, is not expressed in liver (5,6). The human arginase II gene, which maps to chromosome 14q24.1, encodes a 354 amino acid protein (3,5-7). In addition, arginase II contains a putative amino-terminal mitochondrial localization sequence (5,6). |
| APPLICATION DILUTION |
WB(1:300-1000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200) |