alpha Synuclein Polyclonal Antibody
- Size: 100ul
- Application: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
- Reactivity: Human, Mouse, Rat
- Predicted Reactivity: Cow, Pig, Chicken
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Datasheet
Tech Support
IMAGES
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Human U251 cell lysates; Lane 5: Human 293T cell lysates probed with alpha Synuclein Polyclonal Antibody, Unconjugated (bs-0009R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (alpha-Synuclein) polyclonal Antibody, Unconjugated (bs-0009R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-alpha-Synuclein (NT) Polyclonal Antibody, Unconjugated (bs-0009R) 1:300 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with alpha-Synuclein Polyclonal Antibody, Unconjugated (bs-0009R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
PRODUCT DETAILS
SPECIFICATIONS |
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|---|---|
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PRODUCT NAME |
alpha Synuclein Polyclonal Antibody |
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CONJUGATION |
Unconjugated |
|
HOST |
Rabbit |
| SOURCE |
KLH conjugated synthetic peptide derived from human alpha Synuclein |
| IMMUNOGEN RANGE |
51-90/140 |
| CLONALITY |
Polyclonal |
| ISOTYPE |
IgG |
| CONCENTRATION |
1ug/ul |
| PURIFICATION |
Purified by Protein A. |
| STORAGE BUFFER |
Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. |
| STORAGE CONDITION |
Store at -20°C for 12 months.. |
TARGET |
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|---|---|
| GENE ID |
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| SUBCELLULAR LOCATION |
Cytoplasm, Nucleus, Cell membrane |
| SYNONYMS |
NACP; PARK1; PARK4; Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; SNCA |
| BACKGROUND |
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. |
| APPLICATION DILUTION |
ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), ICC(1:100-500) |