Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT



Datasheet      Tech Support









Antibody Testing Data:

Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type VI Collagen-UNLB (SB Cat. No. 1360-01; right) followed by Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT (SB Cat. No. 6300-08), Streptavidin-HRP (SB Cat. No. 7100-05), DAB, and hematoxylin.








Product Details






Product Specifications

Name:

Swine Anti-Goat IgG(H+L), Human/Rat/Mouse SP ads-BIOT

Clone:

Polyclonal

Isotype:

Swine IgG (Isotype Control: -)

Specificity:

Reacts with the heavy and light chains of goat IgG

Format/Conjugate:

BIOT (Biotin)

Buffer Formulation:

Phosphate buffered saline containing 0.1% sodium azide

Concentration:

0.5 mg/mL

Volume:

2.0 mL

Storage & Handling:

2-8°C blue Please refer to product specific SDS.

RRID:

AB_2796285

Recommended Dilutions:

Please refer to product specific Technical Guide.

Applications:

Quality tested applications for relevant formats include -
ELISA
FLISA
Other referenced applications for relevant formats include -
Flow Cytometry 1,2
Immunohistochemistry-Frozen Sections 3
Western Blot 4-7

More information:

Source: Pooled antisera from rabbits hyperimmunized with goat IgG.
Cross Adsorption: Human, rat, and mouse serum proteins (SP); may react with immunoglobulins from other species and the light chains of other goat immunoglobulins.
Purification: Affinity chromatography on goat IgG covalently linked to agarose.

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
















References






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2. Madera-Salcedo IK, Cruz SL, Gonzalez-Espinosa C. Morphine prevents lipopolysaccharide-induced TNF secretion in mast cells blocking IκB kinase activation and SNAP-23 phosphorylation: correlation with the formation of a β-arrestin/TRAF6 complex. J Immunol. 2013;191:3400-9. (FC)

3. Zone JJ, Schmidt LA, Taylor TB, Hull CM, Sotiriou MC, Jaskowski TD, et al. Dermatitis herpetiformis sera or goat anti-transglutaminase-3 transferred to human skin-grafted mice mimics dermatitis herpetiformis immunopathology. J Immunol. 2011;186:4474-80. (IHC-FS)

4. Choi AH, Slamti L, Avci FY, Pier GB, Maira-Litrán T. The pgaABCD locus of Acinetobacter baumannii encodes the production of poly-β-1-6-N-acetylglucosamine, which is critical for biofilm formation. J Bacteriol. 2009;191:5953-63. (WB)

5. Norring SA, Ednie AR, Schwetz TA, Du D, Yang H, Bennett ES. Channel sialic acids limit hERG channel activity during the ventricular action potential. FASEB J. 2013;27:622-31. (WB)

6. Holden JA, Attard TJ, Laughton KM, Mansell A, O'Brien-Simpson NM, Reynolds EC. Porphyromonas gingivalis lipopolysaccharide weakly activates M1 and M2 polarized mouse macrophages but induces inflammatory cytokines. Infect Immun. 2014;82:4190-203. (WB)

7. Pendaries V, Le Lamer M, Cau L, Hansmann B, Malaisse J, Kezic S, et al. In a three-dimensional reconstructed human epidermis filaggrin-2 is essential for proper cornification. Cell Death Dis. 2015;6:e1656. (WB)










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