Rabbit F(ab’)2 Anti-Goat IgG(H+L)-FITC



Datasheet      Tech Support









Antibody Testing Data:

FLISA plate was coated with purified goat IgG. Immunoglobulin was detected with Rabbit F(ab')2 Anti-Goat IgG(H+L)-FITC (SB Cat. No. 6020-02).








Product Details






Product Specifications

Name:

Rabbit F(ab’)2 Anti-Goat IgG(H+L)-FITC

Clone:

Polyclonal

Isotype:

Rabbit F(ab')2 IgG (Isotype Control: Rabbit F(ab')2 IgG-FITC)

Specificity:

Reacts with the heavy and light chains of goat IgG

Format/Conjugate:

FITC (Fluorescein)

Buffer Formulation:

Phosphate buffered saline containing 0.1% sodium azide

Concentration:

0.5 mg/mL

Volume:

1.0 mL

Storage & Handling:

2-8°C; Avoid exposure to light blue Please refer to product specific SDS.

RRID:

AB_2796100

Recommended Dilutions:

Please refer to product specific Technical Guide.

Applications:

Quality tested applications for relevant formats include -
ELISA 1
FLISA
Other referenced applications for relevant formats include -
Immunohistochemistry-Frozen Sections 2,3
Immunocytochemistry 4,5
Western Blot 6

More information:

Source: Pepsin digest of Rabbit Anti-Goat IgG(H+L) (SB Cat. No. 6160).
Cross Adsorption: None; may react with immunoglobulins from other species and the light chains of other goat.

*For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
















References






1. Tang J, Sun X, Yang H, Zhang B, Li Z, Lin Z, et al. Well-oriented ZZ-PS-tag with high Fc-binding onto polystyrene surface for controlled immobilization of capture antibodies. Anal Chim Acta. 2013;776:74-8. (ELISA)

2. Marzari R, Sblattero D, Macor P, Fischetti F, Gennaro R, Marks JD, et al. The cleavage site of C5 from man and animals as a common target for neutralizing human monoclonal antibodies: in vitro and in vivo studies. Eur J Immunol. 2002;32:2773-82. (IHC-FS)

3. Fischetti F, Durigutto P, Macor P, Marzari R, Carretta R, Tedesco F. Selective therapeutic control of C5a and the terminal complement complex by anti-C5 single-chain Fv in an experimental model of antigen-induced arthritis in rats. Arthritis Rheum. 2007;56:1187-97. (IHC-FS)

4. Almeida SR, Aroeira LS, Frymuller E, Dias MÂ, Bogsan CS, Lopes JD, et al. Mouse B-1 cell-derived mononuclear phagocyte, a novel cellular component of acute non-specific inflammatory exudate. Int Immunol. 2001;13:1193-201. (ICC)

5. Popi AF, Zamboni DS, Mortara RA, Mariano M. Microbicidal property of B1 cell derived mononuclear phagocyte. Immunobiology. 2009;214:664-73. (ICC)

6. Li X, Cheng C, Fei M, Gao S, Niu S, Chen M, et al. Spatiotemporal expression of Dexras1 after spinal cord transection in rats. Cell Mol Neurobiol. 2008;28:371-88. (WB)










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