PRODUCT NAME

Bulk anti-Human CD22 antibody (RFB-4) [ICH1179]

SPECIES REACTIVITY

Human

HOST

Mouse

SPECIFICITY

Clone D5 recognizes an epitope on human CD1d

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

CD1d is expressed on monocytes, DCs, macrophages, and epithelial cells

IMMUNOGEN

CD1d a1-a2 domains

CONCENTRATION

FORMULATION

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

PURITY

≥95% monomer by analytical SEC, >95% by SDS Page

ENDOTOXIN

<1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD1d

CLONE

D5

SYNONYMS

BACKGROUND

CD1d antibody, D5, recognizes human CD1d, also known as CD1.1 and Ly-38, a 48 kDa type 1 membrane glycoprotein that belongs to the cluster of differentiation 1 (CD1) family of glycoproteins. CD1d is expressed on epithelial cells and monocyte-derived antigen presenting cells (APCs), including monocytes, DCs, and macrophages. CD1d is an MHC class I-like molecule involved in the presentation of lipid antigens, such as α-galactosylceramide (α-GalCer), to a highly conserved T cell subset, named invariant natural killer T (iNKT) cells or CD1d-restricted T cells1. Following activation, iNKT cells rapidly proliferate and produce large amounts of cytokines and cytolytic mediators

UNIPROT

P15813

PATHOGEN TEST

APPLICATIONS

Immunoprecipitation, Western Blot

APPLICATION NOTES

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.