Home >Ichorbio Antibodies >Anti-Mouse Fas Ligand In Vivo Antibody – Ultra Low Endotoxin (MFL3) [ICH1178]
Anti-Mouse Fas Ligand In Vivo Antibody – Ultra Low Endotoxin (MFL3) [ICH1178]
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Ultra-Low Endotoxin, anti-mouse
- Target: Fas Ligand
- Clone: MFL3
- Host: Armenian Hamster
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Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse Fas Ligand In Vivo Antibody - Ultra Low Endotoxin (MFL3) [ICH1178] |
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SPECIES REACTIVITY |
Mouse |
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HOST |
Armenian Hamster |
| SPECIFICITY |
Clone MFL3 recognizes an epitope on mouse FasL |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
FasL is expressed on activated T cells, NK cells, the eye, and testis |
| IMMUNOGEN |
B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells |
| CONCENTRATION |
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| FORMULATION |
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| PURITY |
>98% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 0.75 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 1% |
| STORAGE CONDITION |
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
Fas Ligand |
| CLONE |
MFL3 |
| SYNONYMS |
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| BACKGROUND |
FasL antibody, clone AFS98, recognizes Fas ligand (FasL), also known as CD178, Apo-1 ligand, and CD95 ligand. FasL is a 40 kDa type II integral membrane protein that belongs to the tumor necrosis factor (TNF) superfamily. FasL is expressed by activated T cells and natural killer (NK cells). Binding of FasL to its receptor Fas (CD95, APO-1) induces apoptotic cell death in Fas-expressing target cells, contributing to anti-viral immunity. FasL also contributes to peripheral tolerance and the downregulation of immune responses through activation-induced autocrine and paracrine T cell death. FasL is also found in the anterior chamber of the eye and on Sertoli cells in the testis, and is implicated in immune-privilege at these sites. FasL also contributes to CD8 proliferation and neutrophil recruitment. Soluble FasL (26 kDa) can be released following cleavage by metalloproteinases and block FasL-mediated signaling. Fas/FasL-signaling is involved in the development of many human diseases, including autoimmunity and cancer. Many human tumors over-express FasL, resulting in tumor infiltrating lymphocyte (TIL) apoptosis and immune evasion, which is associated with poor prognosis. |
| UNIPROT |
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| PATHOGEN TEST |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: |
| APPLICATIONS |
Blocking, Flow Cytometry, Immunofluorescence |
| APPLICATION NOTES |
The suggested concentration for this CD178 antibody, clone MFL3, for staining cells in flow cytometry is ≤ 0.25 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |