PRODUCT NAME

Anti-Mouse MHC Class II In Vivo Antibody - Ultra Low Endotoxin (M5/114.15.2) [ICH1176UL]

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Clone M5/114.15.2 recognizes an epitope on the mouse MHC class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. Clone M5/114.15.2 does not react with I-Af, I-Ak, or I-As.

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

I-Ab/d/q and I-Ed/k are expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells from mice of the I-Ab/d/q and I-Ed/k haplotypes.

IMMUNOGEN

C57BL/6 Spleen Cells

CONCENTRATION

FORMULATION

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

PURITY

>98% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 0.75 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 1%

STORAGE CONDITION

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

MHC Class II

CLONE

M5/114.15.2

SYNONYMS

BACKGROUND

MHC class II antibody, clone M5/114.15.2, recognizes the major histocompatibility complex (MHC) class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. MHC class II is constitutively expressed on professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation. MHC class II consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation and can transduce signals into T cells, enhancing T cell proliferation and activity.

UNIPROT

PATHOGEN TEST

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus

APPLICATIONS

Blocking, CODEX®, Flow Cytometry, IHC (Frozen), Immunoprecipitation

APPLICATION NOTES

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.