Bulk anti-Human CD5 antibody (UCHT-2) [ICH1174]
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-human
- Target: CD5
- Clone: UCHT-2
- Host: Mouse
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Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Bulk anti-Human CD5 antibody (UCHT-2) [ICH1174] |
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SPECIES REACTIVITY |
Human |
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HOST |
Mouse |
| SPECIFICITY |
Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. Anti-Human CD5 recognizes a human T-lymphocyte surface membrane antigen (Mr 67 kDa). |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas. |
| IMMUNOGEN |
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| CONCENTRATION |
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| FORMULATION |
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| PURITY |
≥95% monomer by analytical SEC, >95% by SDS Page |
| ENDOTOXIN |
<1.0 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 5% |
| STORAGE CONDITION |
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
CD5 |
| CLONE |
UCHT-2 |
| SYNONYMS |
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| BACKGROUND |
Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas. |
| UNIPROT |
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| PATHOGEN TEST |
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| APPLICATIONS |
CODEX®, Functional Assays, Flow Cytometry, IHC (Frozen) |
| APPLICATION NOTES |
The suggested concentration for this CD5 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |