Bulk anti-Human CD195 (CCR5) antibody (HEK/1/85a/7a) [ICH1172]
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-human
- Target: CD195
- Clone: HEK/1/85a/7a
- Host: Rat
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Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Bulk anti-Human CD195 (CCR5) antibody (HEK/1/85a/7a) [ICH1172] |
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SPECIES REACTIVITY |
Human |
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HOST |
Rat |
| SPECIFICITY |
Clone HEK/1/85a/7a recognizes an epitope on human CCR5 |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
CCR5 is expressed on memory T cells, macrophages, and immature dendritic cells (DCs) |
| IMMUNOGEN |
CHO cells transfected with full-length human CD195 (CCR5). |
| CONCENTRATION |
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| FORMULATION |
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| PURITY |
>98% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 0.75 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 1% |
| STORAGE CONDITION |
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
CD195 |
| CLONE |
HEK/1/85a/7a |
| SYNONYMS |
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| BACKGROUND |
CCR5 antibody, HEK/1/85a/7a, recognizes C-C chemokine receptor type 5 (CCR5), also known as CD195, CC-CKR-5, CHEMR13, and HIV-1 fusion coreceptor. CCR5 is a 45 kDa seven-transmembrane G-protein-coupled receptor (GPCR). CCR5 is expressed on a subset of memory T cells, macrophages, and immature dendritic cells (DCs) and is upregulated by proinflammatory cytokines. CCR5 binds to inflammatory CC-chemokines, including CCL3 (macrophage inflammatory protein (MIP)-1α), CCL4 (MIP-1β), and RANTES, which increases intracellular calcium levels and results in cytoskeletal rearrangement and chemotactic cell migration. CCR5 is also the coreceptor for HIV-1 following binding of viral gp120 to CD44-7. The levels of CCR5 on cells influence the rate of entry of HIV-1, and patients with lower levels of CCR5 exhibit immunity against infection. CCR5 also contributes to other human diseases, including type I diabetes and cancer. |
| UNIPROT |
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| PATHOGEN TEST |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: |
| APPLICATIONS |
Flow Cytometry, ImmunoCytochemistry |
| APPLICATION NOTES |
The suggested concentration for this CD195 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |