PRODUCT NAME

Anti-Mouse CD70 In Vivo Antibody – Low Endotoxin (TAN 1-7) [ICH1169]

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Clone TAN 1-7 recognizes mouse CD70.

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

CD70 is expressed on activated T Cells, B Cells, and dendritic cells.

IMMUNOGEN

Recombinant mouse CD70 (extracellular domain, aa residues 41-195).

CONCENTRATION

FORMULATION

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD70

CLONE

TAN1-7

SYNONYMS

BACKGROUND

CD70, a cytokine and also known as CD27 ligand, is a member of the tumor necrosis family. As a cytokine, CD70 plays a role in regulating B cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. Non-small cell lung cancer research has shown that CD70 increases the survivability of tumor cells and T cells by signaling through its receptor CD27.

UNIPROT

O55237

PATHOGEN TEST

APPLICATIONS

ELISA, Flow Cytometry, Immunofluorescence, Blocking

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.