PRODUCT NAME

Anti-Mouse DNGR-1 In Vivo Antibody – Ultra Low Endotoxin (1F6) [ICH1168]

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Clone 1F6 recognizes an epitope on mouse DNGR1

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

CD370 (CLEC9A) in mice is expressed on CD8+ DCs and at low levels by plasmacytoid DCs.

IMMUNOGEN

RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope.

CONCENTRATION

FORMULATION

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

PURITY

>98% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 0.75 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 1%

STORAGE CONDITION

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

DNGR-1

CLONE

1F6

SYNONYMS

BACKGROUND

CLEC9A, also known as DNGR1 is defined as a type II membrane protein with a single extracellular C-type lectin domain¹. CLEC9A is involved with adaptive immune response during infection. Additionally, CLEC9A, is being investigated for potential to enhance immune responses produced by vaccines.

UNIPROT

Q8BRU4

PATHOGEN TEST

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for:
Mycoplasma spp.
Mycoplasma pulmonis
Sendai virus
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV1-5)
Theiler’s murine encephalomyelitis virus
Murine norovirus
Reovirus 3
Mouse rotavirus
Ectromelia virus
Lymphocytic choriomeningitis virus
Polyoma virus
Lactate dehydrogenase-elevating virus
Mouse adenovirus (MAD1, MAD2)
Mouse cytomegalovirus
K virus
Mouse thymic virus
Hantaan virus
Corynebacterium bovis
Corynebacterium spp. (HAC2)

APPLICATIONS

Flow Cytometry, Immunofluorescence, Western Blot

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.