PRODUCT NAME

Bulk anti-Human CD64 antibody (10.1) [ICH1167]

SPECIES REACTIVITY

Human

HOST

Mouse

SPECIFICITY

Clone 10.1 recognizes an epitope on human FcγRI

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

FcγRI (CD64) is an IgG receptor expressed by monocytes and myeloid cell lines.

IMMUNOGEN

Rheumatoid synovial fluid cells and fibronectin purified human monocytes

CONCENTRATION

FORMULATION

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD64

CLONE

10.1

SYNONYMS

BACKGROUND

Fc gamma receptors (FcγRs) are membrane-bound proteins which control and mediate communication of IgG antibodies within the immune system. These receptors play a crucial role in the link between the innate and adaptive immune system. FcγRI, CD64, is expressed on dendritic cells, macrophages/monocytes, and granulocytes. Of the FcγRs, FcγRI is capable of interacting with monomeric IgG with relatively high affinity.

UNIPROT

P12314

PATHOGEN TEST

APPLICATIONS

Blocking, Functional Assays, Flow Cytometry, Immunofluorescence, IHC (Frozen), Immunoprecipitation

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.