PRODUCT NAME

Bulk anti-Human CD7 antibody (T3-3A1) [ICH1158]

SPECIES REACTIVITY

Human

HOST

Mouse

SPECIFICITY

Clone T3-3A1 recognizes an epitope on human CD7.

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

CD7 is expressed on T cells, NK cells, thymocytes, hematopoietic progenitors, some normal B cells, malignant B cells, acute lymphocytic leukemia (ALL) cells, and some acute myeloid leukemia (AML) cells. It is weakly expressed on monocytes.

IMMUNOGEN

CONCENTRATION

FORMULATION

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

PURITY

>98% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 0.75 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 1%

STORAGE CONDITION

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD7

CLONE

T3-3A1

SYNONYMS

BACKGROUND

CD7 is a 40 kD type I transmembrane glycoprotein and is a member of the Ig superfamily. It plays a vital role in T-cell interactions, in adition to, T-cell/B-cell interaction during early lymphoid development, and has been shown to interact with PIK3R1. Functional studies indicate that crosslinking of the CD7 can stimulate transmembrane calcium flux in T cells. Lack of CD7 expression can help to diagnose T-cell lymphoproliferative disorders, such as mycosis fungoides/Sezary syndrome and adult T-cell leukemia/lymphoma.

UNIPROT

P50283

PATHOGEN TEST

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for:
Mycoplasma spp.
Mycoplasma pulmonis
Sendai virus
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV1-5)
Theiler’s murine encephalomyelitis virus
Murine norovirus
Reovirus 3
Mouse rotavirus
Ectromelia virus
Lymphocytic choriomeningitis virus
Polyoma virus
Lactate dehydrogenase-elevating virus
Mouse adenovirus (MAD1, MAD2)
Mouse cytomegalovirus
K virus
Mouse thymic virus
Hantaan virus
Corynebacterium bovis
Corynebacterium spp. (HAC2)

APPLICATIONS

Flow cytometry, Immunofluorescence

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.