PRODUCT NAME

Bulk anti-Human CD38 antibody (OKT10) [ICH1157]

SPECIES REACTIVITY

Human

HOST

Mouse

SPECIFICITY

Clone OKT10 recognizes an epitope on human CD38.

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

CD38 is present on the surface of immune cells such as CD4+, CD8+, B lymphocytes, natural killer cells, mature lymphocytes, and mature lymphoplasmacytic cells.

IMMUNOGEN

CONCENTRATION

FORMULATION

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD38

CLONE

OKT10

SYNONYMS

BACKGROUND

CD38 is a 45kD type II transmembrane glycoprotein that plays a role in the regulation of intracellular Ca2+ by synthesizing and hydrolyzing an intracellular calcium ion mobilizing messenger. CD38 has both extracellular and intracellular functions as indicated by the release of soluble protein and the ability of membrane-bound protein to become internalized. The functional molecule is a dimer that contains the catalytic site in the central portion, and is involved in both extracellular and intracellular functions. CD38 plays a role in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a biomarker to measure the progress of a chronic lymphocytic leukemia in patients. Because CD38 is expressed on mature lymphocytes and lymphoplasmacytic cells, OKT10 can be used to study final B cell maturation. OKT10 reactivity with CD38 occurs in an inversely proportional relationship to the occurrence of Ia-like antigenic expression. Hence, these two antigens can be used as reciprocal complementary reactants for the study of mature B cell malignancies, such as CLL, multiple myeloma, and Waldenström malignancy.

UNIPROT

P56528

PATHOGEN TEST

APPLICATIONS

Flow cytometry

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.