Home >Ichorbio Antibodies >Anti-Mouse TCR beta In Vivo Antibody – Ultra Low Endotoxin (H57-597) [ICH1153]
Anti-Mouse TCR beta In Vivo Antibody – Ultra Low Endotoxin (H57-597) [ICH1153]
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Ultra Low Endotoxin, anti-mouse
- Target: TCR Beta
- Clone: H57-597
- Host: Armenian Hamster
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Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse TCR beta In Vivo Antibody – Ultra Low Endotoxin (H57-597) [ICH1153] |
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SPECIES REACTIVITY |
Mouse |
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HOST |
Armenian Hamster |
| SPECIFICITY |
Clone H57-597 recognizes an epitope on the constant region of mouse TCRβ. Clone H57-597 does not cross-react with γδ T cells. |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
TCRβ is expressed on αβ thymocytes, peripheral T cells, and NKT cells. |
| IMMUNOGEN |
Affinity purified TCR from mouse DO-11.10 cells |
| CONCENTRATION |
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| FORMULATION |
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
| PURITY |
>98% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 0.75 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 1% |
| STORAGE CONDITION |
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
TCR Beta |
| CLONE |
H57-597 |
| SYNONYMS |
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| BACKGROUND |
TCRβ antibody, clone H57-597, recognizes the beta chain of the T cell receptor (TCRβ), expressed on αβ thymocytes and T lymphocytes, as well as natural killer T (NKT) cells. TCRβ is a transmembrane protein that consists of an amino-terminal variable region, a constant region, and a stalk segment covalently linked to the TCR alpha chain (TCRα) via a disulfide bond. TCRα/β does not contain signaling domains and is instead noncovalently associated with the CD3 complex (TCR-CD3), essential for TCR signaling. TCRα/β recognizes antigens bound to major histocompatibility complex (MHC) molecules, resulting in phosphorylation of CD3 ITAMs and subsequent T cell activation, proliferation, and survival. |
| UNIPROT |
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| PATHOGEN TEST |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: |
| APPLICATIONS |
Flow Cytometry, Costimulatory, Depletion, Immunoprecipitation, IHC (Frozen), IHC (Paraffin), CODEX |
| APPLICATION NOTES |
The suggested concentration for this TCR β chain (clone H57-597) antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |