PRODUCT NAME

Anti-Mouse TNFR2 In Vivo Antibody - Low Endotoxin (TR75-54.7)

SPECIES REACTIVITY

Mouse

HOST

Armenian Hamster

SPECIFICITY

Anti-TNFR2 In Vivo Antibody - Low Endotoxin (TR75-54.7) recognizes Mouse CD120

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

IMMUNOGEN

Purified Recombinant Mouse TNF R2

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-TNFR2 In Vivo Antibody - Low Endotoxin (TR75-54.7) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

TARGET

TNFR2

CLONE

TR75-54.7

SYNONYMS

BACKGROUND

Tumor necrosis factor receptor II (TNF-RII) is present on most cell types and is considered to play a prominent role in stimulation by TNF-alpha. TNFRII proteins are expressed by hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells. It is expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells

UNIPROT

P25119

PATHOGEN TEST

APPLICATIONS

Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, ELISA

APPLICATION NOTES

Blocking: Clone TR75-54.7 antibody has been reported to block ligand-induced receptor signaling.
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.