Anti-Mouse TNF alpha In Vivo Antibody – Low Endotoxin (TN3-19.12)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-mouse
- Target: TNF alpha
- Clone: TN3-19.12
- Host: Armenian Hamster
-
Datasheet Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
|
---|---|
PRODUCT NAME |
Anti-Mouse TNF alpha In Vivo Antibody - Low Endotoxin (TN3-19.12) |
SPECIES REACTIVITY |
Mouse |
HOST |
Armenian Hamster |
SPECIFICITY |
Anti-TNF alpha In Vivo Antibody - Low Endotoxin (TN3-19.12) recognizes Mouse TNFa |
PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
ANTIGEN DISTRIBUTION |
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells |
IMMUNOGEN |
Recombinant mouse TNFalpha |
CONCENTRATION |
|
FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
PURITY |
>95% by SDS-PAGE and HPLC |
ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
AGGRIGATION |
Aggregation level ≤ 5% |
STORAGE CONDITION |
anti-TNF alpha In Vivo Antibody - Low Endotoxin (TN3-19.12) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
|
---|---|
TARGET |
TNF alpha |
CLONE |
TN3-19.12 |
SYNONYMS |
|
BACKGROUND |
The tumor necrosis factor (TNF-alpha) is a multifaceted polypeptide cytokine known as a mediator of inflammation and immunity. It may mediate some of the significant changes in cellular homeostasis which accompany the invasion of the mammalian host by viruses, bacteria, and parasites. TNF-alpha is an acute phase protein which initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophage and neutrophils to a site of infection. TNF-alpha secreted by the macrophage causes blood clotting which serves to contain the infection. TNF-alpha has been detected in synovial fluid of patients with rheumatoid arthritis. Clone TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-alpha |
UNIPROT |
|
PATHOGEN TEST |
|
APPLICATIONS |
Immunoprecipitation, Western Blot, Functional Assays, Flow Cytometry, Blocking |
APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |