Anti-Mouse IFN alpha In Vivo Antibody – Ultra Low Endotoxin (TIF-3C5)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Ultra Low Endotoxin, anti-mouse
- Target: IFN alpha
- Clone: TIF-3C5
- Host: Armenian Hamster
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Datasheet Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse IFN alpha In Vivo Antibody - Ultra Low Endotoxin (TIF-3C5) |
SPECIES REACTIVITY |
Mouse |
HOST |
Armenian Hamster |
SPECIFICITY |
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PURIFICATION METHOD |
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ANTIGEN DISTRIBUTION |
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IMMUNOGEN |
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CONCENTRATION |
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FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
PURITY |
>98% by SDS-PAGE and HPLC |
ENDOTOXIN |
≤ 0.75 EU/mg as determined by the LAL method |
AGGRIGATION |
Aggregation level ≤ 1% |
STORAGE CONDITION |
anti-IFN alpha In Vivo Antibody - Ultra Low Endotoxin (TIF-3C5) is stable when stored at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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TARGET |
IFN alpha |
CLONE |
TIF-3C5 |
SYNONYMS |
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BACKGROUND |
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UNIPROT |
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PATHOGEN TEST |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: |
APPLICATIONS |
Western Blot, Functional Assays |
APPLICATION NOTES |
Functional Assay: In vitro activity of TIF-3C5 - inhibits IFNalpha -induced (IFNalpha-A, -1, -4, -5, -11 and 13) Stat1 phosphorylation in vitro. [TIF-1D6 does not block in vitro functional activity]. - blocks IFNA (IFNA-A, -1, -4, -5, -11 and 13) induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines in a dose dependent manner. - neutralizes IFNA-induced antiviral activity in vitro following infection of L929 cells with VSV. Functional activity of both recombinant (IFNalpha-A, -1, -4, -5, -11 and 13) and natural IFNalpha can be blocked with no neutralization of IFNgor IFNb. In vivo activity of TIF-3C5 - TIF-3C5 circulates with a half-life of 4 days - blockade of IFNa by TIF-3C5 increases the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of Irf7-/- mice. |
USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
DOCUMENTS
DATASHEET
Datasheet: Anti-Mouse IFN alpha In Vivo Antibody – Ultra Low Endotoxin (TIF-3C5)