PRODUCT NAME

Anti-Mouse, Human CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2)

SPECIES REACTIVITY

Mouse, Human

HOST

Rat

SPECIFICITY

Anti-CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2) recognizes the B-cell determinant (Mr 220 kD) of the CD45 molecule.

PURIFICATION METHOD

ANTIGEN DISTRIBUTION

The CD45R antigen is present on mouse B-cells, B-cell precursors, T cell subset, NK cell subsets .

IMMUNOGEN

Abelson murine leukemia virus-induced pre-B tumor cells

CONCENTRATION

FORMULATION

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

Anti-CD45R In Vivo Antibody - Low Endotoxin (RA3-6B2) is stable for at least four (4) weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD45R

CLONE

RA3-6B2

SYNONYMS

BACKGROUND

CD45R, also known as B220 is a 220 kDa transmembrane protein tyrosine phosphatase expressed on B cells and some subsets of T and NK cells. This antibody is commonly used as a B cell marker as CD45R does play a critical role in TCR and BCR signaling.

UNIPROT

P08575

PATHOGEN TEST

APPLICATIONS

Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Immunofluorescence, ELISA

APPLICATION NOTES

Functional Assay: In vivo treatment with Rat Anti-Mouse CD45R (Clone RA3-6B2) reduces the number of T and B cells and modifies their functional activity.
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.