Anti-Mouse NK1.1 In Vivo Antibody – Ultra Low Endotoxin (PK136)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Ultra Low Endotoxin, anti-mouse
- Target: NK1.1
- Clone: PK136
- Host: Mouse
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Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136) |
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SPECIES REACTIVITY |
Mouse |
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HOST |
Mouse |
| SPECIFICITY |
Anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136) recognizes an epitope on Mouse NK1.1 |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
Mouse NK cells. Endogenous and interferon induced NK cells. Not present on cytotoxic T-cells and Con A stimulated blast cells. May be present on a certain subset of cytotoxic T-cells strains A, AKR, BALB/C, BALB.H-2b, CBA, C3H, DBA/2, 124 were negative using PK136. |
| IMMUNOGEN |
NK-1+ cells from mouse spleen and bone marrow |
| CONCENTRATION |
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| FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| PURITY |
>98% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 0.75 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 1% |
| STORAGE CONDITION |
anti-NK1.1 In Vivo Antibody - Ultra Low Endotoxin (PK136) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
NK1.1 |
| CLONE |
PK136 |
| SYNONYMS |
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| BACKGROUND |
NK-1.1 surface antigen, also known as CD161b/CD161c, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers. |
| UNIPROT |
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| PATHOGEN TEST |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: |
| APPLICATIONS |
Immunoprecipitation, Western Blot, Blocking, Flow Cytometry, CyTOF, IHC (Frozen) |
| APPLICATION NOTES |
Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |