PRODUCT NAME

Anti-Mouse GM-CSF In Vivo Antibody - Ultra Low Endotoxin (MP1-22E9)

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Anti-GM-CSF In Vivo Antibody - Ultra Low Endotoxin (MP1-22E9) recognizes an epitope on Mouse GM-CSF

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype

ANTIGEN DISTRIBUTION

IMMUNOGEN

Yeast Derived Recombinant Mouse GM-CSF

CONCENTRATION

FORMULATION

0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

PURITY

>98% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 0.75 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 1%

STORAGE CONDITION

anti-GM-CSF In Vivo Antibody - Ultra Low Endotoxin (MP1-22E9) is lyophilized and can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

TARGET

GM-CSF

CLONE

MP1-22E9

SYNONYMS

BACKGROUND

Granulocyte-macrophage colony-stimulating factor, often abbreviated to GM-CSF is a pleiotropic cytokine that controls the production and function of blood cells. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF has a critical role in regulation of surfactant homeostasis and alveolar macrophage innate immune functions in the lung.

UNIPROT

P01587

PATHOGEN TEST

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus

APPLICATIONS

Western Blot, ELISA, ELISPOT, Blocking, Immunocytochemistry, IHC (Frozen)

APPLICATION NOTES

Western Blotting: To detect Mouse GM-CSF this monoclonal antibody can be used at a concentration of 1-2 µg/ml.Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.