PRODUCT NAME

Anti-Mouse IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7)

SPECIES REACTIVITY

Mouse

HOST

Mouse

SPECIFICITY

Anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms

PURIFICATION METHOD

This antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

IMMUNOGEN

Plasmid DNA encoding murine IFNb

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>98% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 0.75 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 1%

STORAGE CONDITION

anti-IFN beta In Vivo Antibody - Ultra Low Endotoxin (HDB-4A7) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

IFN beta

CLONE

HDB-4A7

SYNONYMS

BACKGROUND

UNIPROT

PATHOGEN TEST

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for:
Mycoplasma spp.
Mycoplasma pulmonis
Sendai virus
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV1-5)
Theiler's murine encephalomyelitis virus
Murine norovirus
Reovirus 3
Mouse rotavirus
Ectromelia virus
Lymphocytic choriomeningitis virus
Polyoma virus
Lactate dehydrogenase-elevating virus
Mouse adenovirus (MAD1, MAD2)
Mouse cytomegalovirus
K virus
Mouse thymic virus
Hantaan virus
Corynebacterium bovis
Corynebacterium spp. (HAC2)

APPLICATIONS

Western Blot, Blocking, Functional Assays

APPLICATION NOTES

Functional Assay: In Vitro Activity of HDB-4A7 - inhibits IFNb-induced Stat1 phosphorylation (level of inhibition is similar to that seen using anti-IFNAR1 mAb (MAR1-5A3). - blocks IFNb induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines. - neutralizes IFNb-induced antiviral activity in vitro following infection of L929 cells with VSV In Vivo Activity of HDB-4A7 - circulates with a half-life of ~ 14 days. - blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of IFNb-/- mice.
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.