PRODUCT NAME

Anti-Mouse IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7)

SPECIES REACTIVITY

Mouse

HOST

Mouse

SPECIFICITY

Anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7) recognizes an epitope on Mouse IFN-beta and binds specifically to murine IFNbeta and does not bind murine IFNg or several different IFNa species (a-1,4,5 or 13); HDb-4A7 binds both recombinant and natural forms

PURIFICATION METHOD

This antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

IMMUNOGEN

Plasmid DNA encoding murine IFNb

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-IFN beta In Vivo Antibody - Low Endotoxin (HDB-4A7) is stable when stored at 2-8°C. Do not freeze. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

IFN beta

CLONE

HDB-4A7

SYNONYMS

BACKGROUND

UNIPROT

PATHOGEN TEST

APPLICATIONS

Western Blot, Blocking, Functional Assays

APPLICATION NOTES

Blocking: Blocks in vivo IFNb leading to increased the lethality of mice infected with West Nile Virus (WNV), similar to the susceptibility of IFNb-/- mice. Clone HDB-4A7 circulates with a half-life of ~ 14 days.
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.