Anti-Mouse IL-1b In Vivo Antibody – Low Endotoxin (B122)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-mouse
- Target: IL-1b
- Clone: B122
- Host: Armenian Hamster
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Datasheet
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PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse IL-1b In Vivo Antibody - Low Endotoxin (B122) |
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SPECIES REACTIVITY |
Mouse |
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HOST |
Armenian Hamster |
| SPECIFICITY |
Anti-IL-1b In Vivo Antibody - Low Endotoxin (B122) recognizes Mouse IL-1B |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells and many other B cells, T cells, related monocytes |
| IMMUNOGEN |
E. coli -expressed, recombinant mouse IL-1β |
| CONCENTRATION |
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| FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| PURITY |
>95% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 5% |
| STORAGE CONDITION |
anti-IL-1b In Vivo Antibody - Low Endotoxin (B122) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
IL-1b |
| CLONE |
B122 |
| SYNONYMS |
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| BACKGROUND |
Interleukin 1 beta (IL1 beta) is a proinflammatory cytokine belonging to IL1 family that is synthesized as a precursor. The IL1 beta precursor form is not biologically active. It is expressed mainly by stimulated macrophages and monocytes. IL1 beta is known to be a mediator in inflammatory response and to be involved in a variety of cellular activities, proliferation, differentiation and apoptosis. It has been recently found that IL1 beta is a major cause of severe inflammation in the mouse model of Crohns disease which makes IL1 beta an important target for this disease. The B122 antibody has been reported to neutralize the bioactivity of natural or recombinant IL-1β. |
| UNIPROT |
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| PATHOGEN TEST |
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| APPLICATIONS |
Neutralization, Immunoprecipitation, Western Blot, ELISA |
| APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |