Anti-Mouse IL-1a In Vivo Antibody – Low Endotoxin (ALF-161)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-mouse
- Target: IL-1a
- Clone: ALF-161
- Host: Armenian Hamster
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Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse IL-1a In Vivo Antibody - Low Endotoxin (ALF-161) |
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SPECIES REACTIVITY |
Mouse |
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HOST |
Armenian Hamster |
| SPECIFICITY |
Anti-IL-1a In Vivo Antibody - Low Endotoxin (ALF-161) recognizes Mouse IL-1A |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
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| IMMUNOGEN |
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| CONCENTRATION |
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| FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| PURITY |
>95% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 5% |
| STORAGE CONDITION |
anti-IL-1a In Vivo Antibody - Low Endotoxin (ALF-161) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
IL-1a |
| CLONE |
ALF-161 |
| SYNONYMS |
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| BACKGROUND |
Mouse IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. |
| UNIPROT |
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| PATHOGEN TEST |
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| APPLICATIONS |
Western Blot, ELISA, ELISPOT, Neutralization, Immunoprecipitation, Flow Cytometry (Intracellular) |
| APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |