PRODUCT NAME

Anti-Mouse IL-4 In Vivo Antibody - Ultra Low Endotoxin (11B11)

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) recognizes an epitope on Mouse IL-4

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

IMMUNOGEN

Purified Recombinant Mouse IL-4 (>98%)

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>98% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 0.75 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 1%

STORAGE CONDITION

anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) is stable when stored at 2-8°C for 1 week. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

IL-4

CLONE

11B11

SYNONYMS

BACKGROUND

Interleukin-4 (IL-4) is a pleiotropic immune cytokine secreted by activated TH2 cells that inhibits bone resorption. IL-4 is critical for inducing allergic responses. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is also a key regulator in humoral and adaptive immunity. IL-4 is required for the pathogenesis of atopic diseases and immune regulation.

UNIPROT

P07750

PATHOGEN TEST

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus

APPLICATIONS

ELISA Capture, ELISPOT, Flow Cytometry, Neutralization, Immunoprecipitation, Functional Assays, CyTOF, IHC

APPLICATION NOTES

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 µg/ml.
ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-6 µg/ml is recommended.
Neutralization: Anti-Mouse IL-4 (clone 11B11) antibody can neutralize the bioactivity of natural or recombinant IL-4. The Low Endotoxin Functional Formulation purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo.
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.