PRODUCT NAME

Anti-Mouse CTLA-4 In Vivo Antibody - Ultra Low Endotoxin (9H10)

SPECIES REACTIVITY

Mouse

HOST

Syrian Hamster

SPECIFICITY

Anti-CTLA-4 In Vivo Antibody - Ultra Low Endotoxin (9H10) recognizes an epitope on Mouse CTLA-4

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

Activated T cells

IMMUNOGEN

Mouse CTLA-4-human IgG1 fusion protein

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>98% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 0.75 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 1%

STORAGE CONDITION

anti-CTLA-4 In Vivo Antibody - Ultra Low Endotoxin (9H10) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CTLA-4

CLONE

9H10

SYNONYMS

BACKGROUND

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) also known as CD152, is a protein which is expressed on the surface of Helper T cells and plays an important regulatory role in the immune system. CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells. CTLA4 is potentially therapeutic in auto-immune diseases4, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis and may also be useful during organ transplantation and cancer treatment. Clone 9H10 antibody has been shown to promote T cell co-stimulation by blocking CTLA4 binding, allowing for CD28 binding.

UNIPROT

P09793

PATHOGEN TEST

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Hamster parvovirus
Lymphocytic choriomeningitis
Minute virus of mice
Theiler's murine encephalomyelitis virus
Sendai virus
Reovirus 3
Hantaan virus

APPLICATIONS

Western Blot, Blocking, ELISA, Flow Cytometry

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.