Anti-Mouse CD54 In Vivo Antibody – Low Endotoxin (YN1/1.7.4)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-mouse
- Target: CD54
- Clone: YN1/1.7.4
- Host: Rat
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PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) |
SPECIES REACTIVITY |
Mouse |
HOST |
Rat |
SPECIFICITY |
Anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) recognizes an epitope on Mouse ICAM-1 |
PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
ANTIGEN DISTRIBUTION |
The CD54 antigen is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. It has been demonstrated that ICAM-1 enhances T-cell activation. |
IMMUNOGEN |
Mouse NS-1 cells |
CONCENTRATION |
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FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
PURITY |
>95% by SDS-PAGE and HPLC |
ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
AGGRIGATION |
Aggregation level ≤ 5% |
STORAGE CONDITION |
anti-CD54 In Vivo Antibody - Low Endotoxin (YN1/1.7.4) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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TARGET |
CD54 |
CLONE |
YN1/1.7.4 |
SYNONYMS |
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BACKGROUND |
Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies. |
UNIPROT |
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PATHOGEN TEST |
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APPLICATIONS |
Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), Immunoprecipitation |
APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |