PRODUCT NAME

Anti-Mouse CD45 In Vivo Antibody - Low Endotoxin (I3/2.3)

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3) recognizes an epitope on Mouse CD45

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

The CD45 antigen is present on all cells of hematopoietic origin, but not erythrocytes

IMMUNOGEN

Mouse lymphoma cell line

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-CD45 In Vivo Antibody - Low Endotoxin (I3/2.3) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD45

CLONE

I3/2.3

SYNONYMS

BACKGROUND

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA). CD45 plays a key role in TCR and BCR signal transduction.

UNIPROT

P06800

PATHOGEN TEST

APPLICATIONS

Western Blot, Flow Cytometry, IHC (Frozen), IHC (Paraffin)

APPLICATION NOTES

Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume.
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.