Anti-Mouse CD32/CD16 In Vivo Antibody – Low Endotoxin (2.4G2)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-mouse
- Target: CD32/CD16
- Clone: 2.4G2
- Host: Rat
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Datasheet
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PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) |
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SPECIES REACTIVITY |
Mouse |
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HOST |
Rat |
| SPECIFICITY |
Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes. |
| IMMUNOGEN |
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| CONCENTRATION |
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| FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| PURITY |
>95% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 5% |
| STORAGE CONDITION |
anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
CD32/CD16 |
| CLONE |
2.4G2 |
| SYNONYMS |
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| BACKGROUND |
CD16 is a low-affinity IgG Fc receptor III and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. Clone 2.4G2 antibody is used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen-specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo. |
| UNIPROT |
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| PATHOGEN TEST |
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| APPLICATIONS |
Western Blot, Blocking, Flow cytometry, IHC (Frozen), Immunoprecipitation, CODEX |
| APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |