Anti-Mouse CD25 In Vivo Antibody – Ultra Low Endotoxin (PC61)

Anti-Mouse CD25 In Vivo Antibody – Ultra Low Endotoxin (PC61)


Anti-Mouse CD25 In Vivo Antibody – Ultra Low Endotoxin (PC61)

  • Sizes: 5mg, 25mg, 50mg, 100mg,
  • Category: ichorbio, Ultra Low Endotoxin, anti-mouse
  • Target: CD25
  • Clone: PC61
  • Host: Rat

  • Datasheet      Tech Support


Catalog# ICH1054UL

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PRODUCT DETAILS






SPECIFICATIONS

PRODUCT NAME

Anti-Mouse CD25 In Vivo Antibody - Ultra Low Endotoxin (PC61)

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Anti-CD25 In Vivo Antibody - Ultra Low Endotoxin (PC61) recognizes an epitope on Mouse IL-2 RA

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

The CD25 antigen is detected on CD4 positive T-cells but not on CD8 positive T-cells. However, when stimulated with phytohemagglutinin (PHA), the CD25 antigen can be detected on CD8 positive T cells. The CD25 antigen is also expressed on CD20 positive B cells. The CD25 antigen is negative on CD 56 positive natural killer cells, CD 14 positive monocytes, granulocytes, red blood cells and platlets. The CD25 antigen is expressed on malignant cells of adult T-cell leukemias (ATL's) carring HTLV-1, most cases of cutaneous T cell lymphoma and lymphoepithelioid lymphomas among non-Hodgkin's T cell lymphomas.

IMMUNOGEN

IL-2-dependent cytolytic mouse T-cell clone B6.1

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>98% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 0.75 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 1%

STORAGE CONDITION

anti-CD25 In Vivo Antibody - Ultra Low Endotoxin (PC61) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.


TARGET

TARGET

CD25

CLONE

PC61

SYNONYMS

BACKGROUND

IL-2 receptor alpha chain (IL-2Ralpha) is a component of high-affinity IL-2 receptors and thus is a key regulator of lymphocyte proliferation. IL-2R alpha expression occurs at specific stages of early T and B lymphocyte development and is induced upon activation of mature lymphocytes. IL-2R alpha is essential for regulation of both the size and content of the peripheral lymphoid compartment, probably by influencing the balance between clonal expansion and cell death following lymphocyte activation. IL-2Ralpha is also important in the formation of high-affinity binding sites, and the inducibility of the alpha gene contributes to the highly regulated and transient display of high-affinity IL-2R.

UNIPROT

P01590

PATHOGEN TEST

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus

APPLICATIONS

Immunoprecipitation, Western Blot, Functional Assays, Blocking, Flow Cytometry, IHC (Frozen)

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.












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