Anti-Mouse CD11b In Vivo Antibody – Low Endotoxin (M1/70)

Anti-Mouse CD11b In Vivo Antibody – Low Endotoxin (M1/70)


Anti-Mouse CD11b In Vivo Antibody – Low Endotoxin (M1/70)

  • Sizes: 5mg, 25mg, 50mg, 100mg,
  • Category: ichorbio, Low Endotoxin, anti-mouse
  • Target: CD11b
  • Clone: M1/70
  • Host: Rat

  • Datasheet      Tech Support


Catalog# ICH1049

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PRODUCT DETAILS






SPECIFICATIONS

PRODUCT NAME

Anti-Mouse CD11b In Vivo Antibody - Low Endotoxin (M1/70)

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70) recognizes the (Mr 170 kD) alpha m subunit of Mac-1 (CD11b/CD18, alpha m beta 2 integrin), the mouse macrophage-granulocyte specific antigen. Mac-1 is a macrophage differentiation antigen associated with type three complement receptor and mediates adhesion to CD54 (ICAM-1).

PURIFICATION METHOD

ANTIGEN DISTRIBUTION

The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes.

IMMUNOGEN

B10 mouse spleen cells enriched for T cells

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-CD11b In Vivo Antibody - Low Endotoxin (M1/70) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.


TARGET

TARGET

CD11b

CLONE

M1/70

SYNONYMS

BACKGROUND

UNIPROT

P05555

PATHOGEN TEST

APPLICATIONS

Immunoprecipitation, Western Blot, Functional Assays, Flow Cytometry, IHC (Frozen), CyTOF, Immunocytochemistry

APPLICATION NOTES

Functional Assay: Activation of mouse peritoneal macrophages
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.












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