Anti-Mouse CD8 beta In Vivo Antibody – Ultra Low Endotoxin (53-5.8)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Ultra Low Endotoxin, anti-mouse
- Target: CD8 beta
- Clone: 53-5.8
- Host: Rat
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Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse CD8 beta In Vivo Antibody - Ultra Low Endotoxin (53-5.8) |
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SPECIES REACTIVITY |
Mouse |
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HOST |
Rat |
| SPECIFICITY |
Anti-CD8 beta In Vivo Antibody - Ultra Low Endotoxin (53-5.8) recognizes an epitope on Mouse CD8b.2 |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1 and is found on the majority of thymocytes, cytotoxic T cell subsets |
| IMMUNOGEN |
Mouse thymus or spleen |
| CONCENTRATION |
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| FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.4 and does not contain BSA or sodium azide as a preservative. |
| PURITY |
>98% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 0.75 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 1% |
| STORAGE CONDITION |
anti-CD8 beta In Vivo Antibody - Ultra Low Endotoxin (53-5.8) is stable when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
CD8 beta |
| CLONE |
53-5.8 |
| SYNONYMS |
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| BACKGROUND |
CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. |
| UNIPROT |
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| PATHOGEN TEST |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: |
| APPLICATIONS |
Western Blot, Functional Assays, Flow Cytometry, Immunocytochemistry, IHC (Frozen), Immunoprecipitation |
| APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |