PRODUCT NAME

Anti-Mouse CD8 In Vivo Antibody - Low Endotoxin (YTS-169)

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Anti-CD8 In Vivo Antibody - Low Endotoxin (YTS-169) recognizes an epitope on Mouse CD8

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

Present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells. CD8 participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck.

IMMUNOGEN

CBA mouse thymocytes

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

<0.5 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-CD8 In Vivo Antibody - Low Endotoxin (YTS-169) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD8

CLONE

YTS-169

SYNONYMS

BACKGROUND

UNIPROT

PATHOGEN TEST

APPLICATIONS

IHC (Frozen), In vivo Depletion, Flow Cytometry

APPLICATION NOTES

Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections but the concentration is assay dependent.
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.