Anti-Mouse CD4 In Vivo Antibody – Low Endotoxin (GK1.5)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-mouse
- Target: CD4
- Clone: GK1.5
- Host: Rat
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PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse CD4 In Vivo Antibody - Low Endotoxin (GK1.5) |
SPECIES REACTIVITY |
Mouse |
HOST |
Rat |
SPECIFICITY |
Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4 |
PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
ANTIGEN DISTRIBUTION |
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines. |
IMMUNOGEN |
Mouse CTL clone V4 |
CONCENTRATION |
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FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
PURITY |
>95% by SDS-PAGE and HPLC |
ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
AGGRIGATION |
Aggregation level ≤ 5% |
STORAGE CONDITION |
anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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TARGET |
CD4 |
CLONE |
GK1.5 |
SYNONYMS |
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BACKGROUND |
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation. |
UNIPROT |
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PATHOGEN TEST |
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APPLICATIONS |
Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation |
APPLICATION NOTES |
Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation. |
USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |