Anti-Mouse CD3 In Vivo Antibody – Low Endotoxin (17A2)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-mouse
- Target: CD3
- Clone: 17A2
- Host: Rat
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PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Anti-Mouse CD3 In Vivo Antibody - Low Endotoxin (17A2) |
SPECIES REACTIVITY |
Mouse |
HOST |
Rat |
SPECIFICITY |
Anti-CD3 In Vivo Antibody - Low Endotoxin (17A2) recognizes an epitope on Mouse CD3. The 17A2 clone reacts with the epsilon and gamma chains and not delta nor zeta of CD3. |
PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
ANTIGEN DISTRIBUTION |
The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested. |
IMMUNOGEN |
gamma/delta TCR-positive T-T hybridoma D1 |
CONCENTRATION |
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FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
PURITY |
>95% by SDS-PAGE and HPLC |
ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
AGGRIGATION |
Aggregation level ≤ 5% |
STORAGE CONDITION |
anti-CD3 In Vivo Antibody - Low Endotoxin (17A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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TARGET |
CD3 |
CLONE |
17A2 |
SYNONYMS |
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BACKGROUND |
CD3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR A/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
UNIPROT |
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PATHOGEN TEST |
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APPLICATIONS |
Immunoprecipitation, Western Blot, Flow Cytometry, IHC (Frozen), Immunocytochemistry |
APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |