PRODUCT NAME

Anti-Mouse CD86 In Vivo Antibody - Low Endotoxin (GL1)

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) recognizes an epitope on Mouse CD86

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

IMMUNOGEN

LPS-activated CBA/Ca mouse splenic B cells

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD86

CLONE

GL1

SYNONYMS

BACKGROUND

CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the Ig gene superfamily. CD86 is a ligand for CD28 and CD152 (CTLA-4). CD86 is related to the CD80 molecule and functions as a counterreceptor for CD28 and CTLA-4. The GL1 clone has been shown to provide critical signals for T-Cell-dependent responses in vivo and in vitro.

UNIPROT

P42082

PATHOGEN TEST

APPLICATIONS

Flow Cytometry, Immunoprecipitation, IHC (Frozen), Immunocytochemistry, Blocking, Immunofluorescence, Western Blot, CyTOF

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.