PRODUCT NAME

Anti-Mouse 4-1BB In Vivo Antibody - Ultra Low Endotoxin (3H3)

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Anti-4-1BB In Vivo Antibody - Ultra Low Endotoxin (3H3) recognizes an epitope on Mouse 4-1BB

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

Activated B cells and T cells, macrophages, and dendritic cells

IMMUNOGEN

Unknown

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.4, containing no preservatives.

PURITY

>98% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 0.75 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 1%

STORAGE CONDITION

anti-4-1BB In Vivo Antibody - Ultra Low Endotoxin (3H3) is stable when stored at 2-8°C. For long term storage it is recommended to store at -80°C and avoid repeat freeze thaw cycles.

TARGET

4-1BB

CLONE

3H3

SYNONYMS

BACKGROUND

Anti-Mouse CD137/4-1BB clone 3H3 is a monoclonal antibody which reacts with mouse 4-1BB, a TNF receptor superfamily member also known as CD137. The 3H3 antibody has been shown to stimulate 4-1BB signaling in vivo.

UNIPROT

P20334

PATHOGEN TEST

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus

APPLICATIONS

Western Blot, Functional Assays, ELISA, Stimulation, Immunoprecipitation

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.