PRODUCT NAME

Anti-Mouse 4-1BB In Vivo Antibody - Low Endotoxin (3H3)

SPECIES REACTIVITY

Mouse

HOST

Rat

SPECIFICITY

Anti-4-1BB In Vivo Antibody - Low Endotoxin (3H3) recognizes an epitope on Mouse 4-1BB

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

Activated T cells and B cells

IMMUNOGEN

Unknown

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-4-1BB In Vivo Antibody - Low Endotoxin (3H3) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

4-1BB

CLONE

3H3

SYNONYMS

BACKGROUND

4-1BB (CD137) is a costimulatory molecule expressed on the surface of activated T cells and plays a role in T-cell-mediated proliferative response. 4-1BB signal regulates CD28 co-stimulation in the targeted subset cells to favor Th1 development and maintain long-term cell growth. 4-1BB is also expressed on dendritic cells (DCs) and controls the duration, DC-T interaction, and, therefore, immunogenicity. Clone 3H3 antibody is an agonistic antibody that has been shown to stimulate 4-1BB signaling in vivo.

UNIPROT

P20334

PATHOGEN TEST

APPLICATIONS

Western Blot, Functional Assays, ELISA, Stimulation, Immunoprecipitation

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.