Bulk anti-Human CD119 antibody (GIR 208)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-human
- Target: CD119
- Clone: GIR 208
- Host: Mouse
-
Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
|
|---|---|
|
PRODUCT NAME |
Bulk anti-Human CD119 antibody (GIR 208) |
|
SPECIES REACTIVITY |
Human |
|
HOST |
Mouse |
| SPECIFICITY |
Anti-CD119 In Vivo Antibody - Low Endotoxin (GIR 208) recognizes Human IFN gamma R alpha |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
The IFN-gamma receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes. |
| IMMUNOGEN |
Human IFN-gamma R alpha, Purified from human placenta |
| CONCENTRATION |
|
| FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| PURITY |
>95% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 5% |
| STORAGE CONDITION |
anti-CD119 In Vivo Antibody - Low Endotoxin (GIR 208) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
|
|---|---|
| TARGET |
CD119 |
| CLONE |
GIR 208 |
| SYNONYMS |
|
| BACKGROUND |
Interferon-gamma (IFN-gamma) exerts its biologic effects by interacting with a single high affinity receptor, IFN-gamma receptor. This receptor is present on almost all cell types except mature erythrocytes. The IFN-gamma receptor consists of two polypeptide chains alpha and beta and requires association of JAK1, JAK2 and Stat1 for IFN-gamma signal transduction. In signal transduction, IFN-gamma induces tyrosine phosphorylation of IFN-gamma receptor alpha (IFNGR1) leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-gamma induced gene transcription. The IFN-gamma receptor is structurally related to the recently cloned IL-10 receptor. GIR 208 binds IFNGR1 and blocks binding of IFN-gamma to the receptor. |
| UNIPROT |
|
| PATHOGEN TEST |
|
| APPLICATIONS |
Immunoprecipitation, Western Blot, ELISA, Neutralization, Flow Cytometry |
| APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |