PRODUCT NAME

Bulk anti-Human HLA-DR antibody (L243)

SPECIES REACTIVITY

Human

HOST

Mouse

SPECIFICITY

Anti-HLA-DR In Vivo Antibody - Low Endotoxin (L243) recognizes/reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross-react with HLA-DP and HLA-DQ

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

The HLA-DR antigen is present on B-lymphocytes, activated T-lymphocytes and activated natural killer (NK) lymphocytes. This antigen is also expressed on monocytes, macrophages, human progenitor cells and B-cell lymphomas.

IMMUNOGEN

Unknown

CONCENTRATION

FORMULATION

No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-HLA-DR In Vivo Antibody - Low Endotoxin (L243) is stable for at least four (4) weeks when stored at 2-8°C. For long-term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

TARGET

HLA-DR

CLONE

L243

SYNONYMS

BACKGROUND

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD (heavy) chain & a 27 kD (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, and dendritic cells. HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Clone L243 binds a conformational epitope on HLA-DRalpha which depends on the correct folding of the Aβ heterodimer.

UNIPROT

PATHOGEN TEST

APPLICATIONS

Western Blot, Blocking, Flow Cytometry, Immunoprecipitation, CyTOF, IHC (Frozen)

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.