PRODUCT NAME

Bulk anti-Human CD71 antibody (T56/14)

SPECIES REACTIVITY

Human

HOST

Mouse

SPECIFICITY

Anti-CD71 In Vivo Antibody - Low Endotoxin (T56/14) recognizes Human CD71 transferrin receptor (Mr 190 kDa)

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

The transferrin receptor is a cell surface glycoprotein related to cell proliferation. Up-regulation of the CD71 antigen is associated with actively dividing cells. Transferrin receptors are also present on reticulocytes, but are lost during maturation into erythrocytes.

IMMUNOGEN

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

<1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-CD71 In Vivo Antibody - Low Endotoxin (T56/14) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD71

CLONE

T56/14

SYNONYMS

BACKGROUND

Anti-transferrin receptor may be used to identify activated lymphocytes. The CD71 antibody may be used in studies of erythropoiesis.

UNIPROT

P02786

PATHOGEN TEST

APPLICATIONS

Flow Cytometry, Western Blot, Immunoprecipitation

APPLICATION NOTES

Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.