PRODUCT NAME

Bulk anti-Human CD56 antibody (ERIC-1)

SPECIES REACTIVITY

Human

HOST

Mouse

SPECIFICITY

Anti-CD56 In Vivo Antibody - Low Endotoxin (ERIC-1) recognizes Human CD56. Anti-Human CD56 recognizes the (Mr 180, 145, 125 kDa) isoforms of the human neural cell adhesion molecule (NCAM)

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

The CD56 (NCAM) antigen is expressed on most neuroectodermal derived cell lines, tissues, and tumors. NCAM is also known to be expressed on some mesodermally derived tissues such as muscle and on natural killer (NK) lymphocytes. The binding of ERIC-1 to both normal and neoplastic tissue is lost when tissues are conventionally fixed in formalin and embedded in paraffin. The epitope was preserved when exposed to dehydrating fixatives such as cold acetone (-20EC) for 5 min. CD56 is present on 10-25% of peripheral blood lymphocytes.

IMMUNOGEN

Human Retinoblastoma tumor

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

<0.5 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-CD56 In Vivo Antibody - Low Endotoxin (ERIC-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

TARGET

CD56

CLONE

ERIC-1

SYNONYMS

BACKGROUND

Anti-Human CD56 can be used in studies of the neural cell adhesion molecule. This antibody may also be used to study isoforms of NCAM expressed on different tumor types. Anti-CD56 (clone ERIC-1) works well in western blot for analysis of isoforms expressed. Anti-CD56 did not react with any of the leukemias or lymphomas tested

UNIPROT

P13591

PATHOGEN TEST

APPLICATIONS

ELISA Capture, IHC (Frozen), IHC (Paraffin), Western Blot, Immunoprecipitation, Dot Blot

APPLICATION NOTES

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 80 ng/mL.
Dot Blots: Immunoblotting: Recognizes 120, 140, and 180 kDa NCAM isoforms.
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.