Bulk anti-Human CD20 antibody (2H7)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Ultra Low Endotoxin, anti-human
- Target: CD20
- Clone: 2H7
- Host: Mouse
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Datasheet
Tech Support
PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Bulk anti-Human CD20 antibody (2H7) |
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SPECIES REACTIVITY |
Human |
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HOST |
Mouse |
| SPECIFICITY |
Anti-CD20 In Vivo Antibody - Ultra Low Endotoxin (2H7) recognizes an epitope on Human CD20 |
| PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
| ANTIGEN DISTRIBUTION |
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| IMMUNOGEN |
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| CONCENTRATION |
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| FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| PURITY |
>98% by SDS-PAGE and HPLC |
| ENDOTOXIN |
≤ 0.75 EU/mg as determined by the LAL method |
| AGGRIGATION |
Aggregation level ≤ 1% |
| STORAGE CONDITION |
anti-CD20 In Vivo Antibody - Ultra Low Endotoxin (2H7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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| TARGET |
CD20 |
| CLONE |
2H7 |
| SYNONYMS |
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| BACKGROUND |
The CD20 antigen, also called MS4A1, B1 and Bp35, is a non-glycosylated, transmembrane spanning, phosphoprotein. The CD20 protein is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells, and is synchronous with the expression of surface IgM. It is also expressed in some follicular dendritic cells and at low levels on a T cell subset. CD20 has been shown to be heavily phosphorylated in activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn), and exists in a complex with MHC class I and II, CD53, CD81, and CD82. Extensive CD20 cross-linking may also influence cell death by apoptosis. Therapy with antibodies against CD20 is a widely used regimen in the treatment of all B cell lymphomas and leukemias. |
| UNIPROT |
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| PATHOGEN TEST |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: |
| APPLICATIONS |
Flow Cytometry, CyTOF, IHC (Frozen), Immunoprecipitation |
| APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
| USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |