PRODUCT NAME

Bulk anti-Human CD16 antibody (3G8)

SPECIES REACTIVITY

Human

HOST

Mouse

SPECIFICITY

Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16

PURIFICATION METHOD

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

ANTIGEN DISTRIBUTION

CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans.

IMMUNOGEN

Human PMN cells

CONCENTRATION

FORMULATION

0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide added.

PURITY

>95% by SDS-PAGE and HPLC

ENDOTOXIN

≤ 1.0 EU/mg as determined by the LAL method

AGGRIGATION

Aggregation level ≤ 5%

STORAGE CONDITION

anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is stable when stored at 2-8°C.

TARGET

CD16

CLONE

3G8

SYNONYMS

BACKGROUND

CD16 is known as low affinity IgG receptor III (Fc gamma RIII) and is expressed as two distinct forms (CD16a and CD16b). CD16a (Fc gamma RIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (Fc gamma RIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils.

UNIPROT

P08637

PATHOGEN TEST

APPLICATIONS

IHC (Frozen), Flow Cytometry, Immunoprecipitation, Blocking, CyTOF

APPLICATION NOTES

Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume.
Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation.
Each investigator should determine their own optimal working dilution for specific applications.

USAGE

Products are for research use only. Not for use in diagnostic or therapeutic procedures.