Bulk anti-Human CD11b antibody (ICRF44)
- Sizes: 5mg, 25mg, 50mg, 100mg,
- Category: ichorbio, Low Endotoxin, anti-human
- Target: CD11b
- Clone: 44
- Host: Mouse
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PRODUCT DETAILS
SPECIFICATIONS |
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PRODUCT NAME |
Bulk anti-Human CD11b antibody (ICRF44) |
SPECIES REACTIVITY |
Human |
HOST |
Mouse |
SPECIFICITY |
Anti-CD11b In Vivo Antibody - Low Endotoxin (44) recognizes Human CD11b. Anti-Human CD11b recognizes the alpha subunit of the CD11b/CD18 complex, an alpha/Beta heterodimeric surface glycoprotein on human leukocytes (Mr 165-170 kDa). The CD11b/CD18 antigen belongs to the B2 integrin family. Other names include Mac-1, Cr3, MO-1 and C3bi receptor. The CD11b molecule functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. |
PURIFICATION METHOD |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
ANTIGEN DISTRIBUTION |
The CD11b antigen is expressed on approximately 30% of human peripheral blood lymphocytes, most natural killer lymphocytes, circulating monocytes, granulocytes, a subset of T-cells and subsets of macrophages. |
IMMUNOGEN |
Unknown |
CONCENTRATION |
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FORMULATION |
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
PURITY |
>95% by SDS-PAGE and analyzed by silver stain. |
ENDOTOXIN |
≤ 1.0 EU/mg as determined by the LAL method |
AGGRIGATION |
Aggregation level ≤ 5% |
STORAGE CONDITION |
anti-CD11b In Vivo Antibody - Low Endotoxin (44) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
TARGET |
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TARGET |
CD11b |
CLONE |
44 |
SYNONYMS |
|
BACKGROUND |
Anti-CD11b may be used to study lymphocyte subsets in flow cytometry and in cell adhesion research (Mac-1). The antibody may be used to stain tissue sections which have been acetone fixed and frozen. |
UNIPROT |
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PATHOGEN TEST |
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APPLICATIONS |
Flow Cytometry, Western Blot, IHC (Frozen), Immunoprecipitation |
APPLICATION NOTES |
Each investigator should determine their own optimal working dilution for specific applications. |
USAGE |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |